Potensi Ekstrak Etanol Daun Pare (Momordica charantia L.) sebagai Alternatif Obat Penyembuh Luka pada Punggung Mencit Jantan (Mus musculus L.)

Muhammad Pazry, Hendri Busman, Nuning Nurcahyani, Sutyarso Sutyarso

Abstract


The leaves of bitter melon (Momordica charantia L.) contains flavonoid, tannin and saponin which plays a role in wound healing.  The purpose of this study was to determine the effect of bitter melon leaves (M. charantia L.) ethanolic extract on wound healing the back injury on male mice. The parameters of this study were the length of wound closure and histopathological observations by looking at the level of epithelialization, the number of new blood vessels, and the number of inflammatory cells.This study was conducted in a complete randomized design. The mice were divided into five groups: [K (+)], [K (-)], [ P1], [P2], [P3], each group consist four mices. All of mice was wounded by cut on the back skin about 1,5 cm in length. The wound were treated every two times a day for 9 days, K(+) is given povidone iodine as a positive control, K(-) is given 95% ethanol as a negative control, P1 is given 50% ethanolic extract of bitter melon, P2 is given 75% ethanolic extract of bitter melon and P3 is given 100% ethanolic extract of bitter melon. On the 10th day, one of the mice from all of groups was taken to observe the histophatology of their skin. The results showed that the leaf extract of bitter melon has wound healing potential by accelerate the healing process of back skin’s wound in miceand has best histopathology  picture compared to controls.


Keywords


Bitter Melon (Momordica charantia L.), Male Mice (Mus musculus L.), povidone iodine

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References


Ajizah, A. 2004. Sensitivitas Salmonella typhimurium Terhadap Ekstrak Daun Jambu Biji (Psidium guajava) Bioscientiae. Volume 1, No. 1. Program Studi Biologi FMIPA Universitas Lambung Mangkurat.

Faure, D. 2002. The family-3 glycoside hydrolises: from housekeeping function to host-microbe interction. Appled and Environmental Microbiology 64(4):1485-1490.

Franz, M.G., M.C. Robson., D.L. Steed., A.B. Barbul., C. Harold., M. Diane. 2008. Guidelines to aid healing of acute wounds by decreasing impediments of healing. Wound Rep and Regenerat. 16(6): 723–48.

Klokke. 1980. Pedoman Untuk Pengobatan Luar Penyakit Kulit. Jakarta.: PT. Gramedia.

MacKay, D. And A.L. Miller. 2003. Nutritional Support for Wound Healing, Alternative Medicine Review, 8, 369-370.

Permadi, A. 2008. Membuat Kebun Tanaman Obat. Jakarta : Pustaka Bunda.

Pongsipulung, G. 2012. Formulasi dan Pengujian Salep Ekstrak Bonggol Pisang

Ambon (Musa paradisiaca var. sapientum L) Terhadap Luka Terbuka Pada Kulit Tikus Putih Jantan Galur Wistar (Rattus norvegicus). (Skripsi). FMIPA Universitas Sam Ratulangi, Manado.

Sammartino, G., M. Tia., S. Tete., L. Perillo., O. Trosino. 2012. Adverse reaction to irrigation with povidone-iodine after deep-impacted, lower third molar extraction. J Biol Regul Homeost Agents. 26(1): 145–9.

Subahar, T. 2004. Khasiat dan Manfaat Pare, si Pahit Pembasmi Penyakit : Agromedia Pustaka. Jakarta. 4-16, 45-46.

Wijaya, B.A. 2014. Potensi Ekstrak Tangkai Daun Talas (Colocasia esculenta L.) Sebagai Alternatif Obat Luka Pada Kulit Kelinci (Oryctolagus cuniculus). Jurnal Ilmiah Farmasi – UNSRAT. 3(3).


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